Analgesic and anti-inflammatory effects of Crinum asiaticum leaf alcoholic extract in animal models

This study investigated the analgesic and anti-inflammatory effects of Crinum asiaticum (Amaryllidaceae) leaf ethanolic extract. Analgesic effect was investigated in acetic acid induced writhing model and formalin induced licking model in swiss albino mice. Anti-inflammatory effect was conducted in carrageenan-induced paw edema model of albino rat. Data were analyzed by one-way analysis of variance (ANOVA) followed by post hoc multiple comparison test. In analgesic study, C. asiaticum extract inhibited 42.34±3.20% of acetic acid induced pain at higher dose of 2.0 g/kg body weight. The effect was statistically significant (p<0.001) compared to the positive control, diclofenac sodium (10 mg/kg). The extract reduced the formalin induced pain 22.60±1.39% in early phase and 27.11±0.87% in late phase at the same dose of 2.0 g/kg and the reductions were significant (p<0.01) compared to the positive control morphine (0.5 mg/kg). In a time-dependent inhibition of carrageenaninduced paw edema model, the extract promoted the inhibitions of paw edema 51.60±2.50% at the 1 st h and 40.80±0.52% at the 4 th h of administration. These inhibitions were also significant (p<0.01) in comparison to those promoted by diclofenac sodium. No mortality was observed in acute toxicity test. The study concludes that C. asiaticum leaf extract has potential analgesic and anti-inflammatory effects to be recorded as plant-derived complementary medicine.